Endowing Universal CAR T-cell with Immune-Evasive Properties using TALEN-Gene Editing.

Jo, S.; Das, S.; Williams, A.; Chretien, A.-s.; Pagliardini, T.; Leroy, A.; postigo-fernandez, j.; leclerre, D.; jahangiri, B.; chion-sotinel, i.; Gouble, A.; Dusseaux, M.; Galetto, r.; Duclert, a.; Marcenaro, E.; Devillier, R.; Olive, D.; Duchateau, p.; Poirot, L.; Valton, J.

doi:10.1101/2021.12.06.471451

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bioRxiv · 10.1101/2021.12.06.471451

Endowing Universal CAR T-cell with Immune-Evasive Properties using TALEN-Gene Editing.

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Abstract

Universal CAR T-cell therapies are poised to revolutionize cancer treatment and to improve patient outcomes. However, realizing these advantages in an allogeneic setting requires universal CAR T-cells that can kill target tumor cells, avoid depletion by the host immune system, and proliferate without attacking host tissues. Here, we describe the development of a novel immune-evasive CAR T-cells scaffold that evades NK cell and alloresponsive T-cell attacks and imparts efficient antitumor activity in vitro and in vivo. This scaffold could enable the broad use of universal CAR T-cells in allogeneic settings and holds great promise for future powerful clinical applications.

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Jo, S., Das, S., Williams, A., Chretien, A.-s., Pagliardini, T., Leroy, A., postigo-fernandez, j., leclerre, D., jahangiri, B., chion-sotinel, i., Gouble, A., Dusseaux, M., Galetto, r., Duclert, a., Marcenaro, E., Devillier, R., Olive, D., Duchateau, p., Poirot, L., Valton, J.. 2021-12-06. Endowing Universal CAR T-cell with Immune-Evasive Properties using TALEN-Gene Editing.. https://doi.org/10.1101/2021.12.06.471451

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