Sociodemographic effects on immune cell composition in a free-ranging non-human primate.

Rosado, M. R. S.; Marzan-Rivera, N.; Watowich, M. M.; Pantoja, P.; Pavez-Fox, M. A.; Siracusa, E. R.; Negron-Del Valle, J. E.; Phillips, D.; Ruiz-Lambides, A.; Cayo Biobank Research Unit,; Martinez, M. I.; Montague, M. J.; Platt, M. L.; Higham, J. P.; Brent, L. J. N.; Sariol, C. A.; Snyder-Mackler, N.

doi:10.1101/2021.12.06.471383

bioRxiv · 10.1101/2021.12.06.471383

Sociodemographic effects on immune cell composition in a free-ranging non-human primate.

Rosado, M. R. S.·Marzan-Rivera, N.·Watowich, M. M.·Pantoja, P.·Pavez-Fox, M. A.·Siracusa, E. R.·Negron-Del Valle, J. E.·Phillips, D.·Ruiz-Lambides, A.·Cayo Biobank Research Unit,·Martinez, M. I.·Montague, M. J.·Platt, M. L.·Higham, J. P.·Brent, L. J. N.·Sariol, C. A.·Snyder-Mackler, N.

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Abstract

Increasing age is associated with dysregulated immune function and increased inflammation- patterns that are also observed in individuals exposed to chronic social adversity. Yet we still know little about how social adversity impacts the immune system and how it might promote age-related diseases. Here, we investigated how immune cell diversity varied with age, sex and social adversity (operationalized as low social status) in free-ranging rhesus macaques. We found age-related signatures of immunosenescence, including lower proportions of CD20+ B cells, CD20+/CD3+ ratio, and CD4+/CD8+ T cell ratio - all signs of diminished antibody production. Age was associated with higher proportions of CD3+/CD8+ Cytotoxic T cells, CD16+/CD3-Natural Killer cells, CD3+/CD4+/CD25+ and CD3+/CD8+/CD25+ T regulatory cells, and CD14+/CD16+/HLA-DR+ intermediate monocytes, and lower levels of CD14+/CD16-/HLA-DR+ classical monocytes, indicating greater amounts of inflammation and immune dysregulation. We also found an effect of exposure to social adversity (i.e., low social status) that was sex-dependent. High-status males, relative to females, had higher CD20+/CD3+ ratios and CD16+/CD3 Natural Killer cell proportions, and lower proportions of CD8+ Cytotoxic T cells. Further, low status females had higher proportions of cytotoxic T cells than high status females, while the opposite was observed in males. High status males had higher CD20+/CD3+ ratios than low status males. Together, our study identifies immune cell types that differ by age in a human-relevant primate model animal, and demonstrates a novel link between sex-dependent immunity and social adversity.

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Rosado, M. R. S., Marzan-Rivera, N., Watowich, M. M., Pantoja, P., Pavez-Fox, M. A., Siracusa, E. R., Negron-Del Valle, J. E., Phillips, D., Ruiz-Lambides, A., Cayo Biobank Research Unit,, Martinez, M. I., Montague, M. J., Platt, M. L., Higham, J. P., Brent, L. J. N., Sariol, C. A., Snyder-Mackler, N.. 2021-12-07. Sociodemographic effects on immune cell composition in a free-ranging non-human primate.. https://doi.org/10.1101/2021.12.06.471383

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