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bioRxiv · 10.1101/2021.11.30.470571

CADHERIN mediated AMIS localisation

Liang, X.·Weberling, A.·Hii, C. Y.·Zernicka-Goetz, M.·Buckley, C. E.
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Abstract

Individual cells within de novo polarising tubes and cavities must integrate their forming apical domains into a centralised apical membrane initiation site (AMIS). This is necessary to enable organised lumen formation within multi-cellular tissue. Despite the well documented importance of cell division in localising the AMIS, we have found a division-independent mechanism of AMIS localisation that relies instead on Cadherin-mediated cell-cell adhesion. Our study of de novo polarising mouse embryonic stem cells (mESCs) cultured in 3D suggest that cell-cell adhesion localises apical proteins such as PAR-6 to a centralised AMIS. Unexpectedly, we also found that mESC cell clusters lacking functional E-cadherin still formed a lumen-like cavity in the absence of AMIS localisation but did so at a later stage of development via a closure mechanism, instead of via hollowing. This work suggests that there are two, interrelated mechanisms of apical polarity localisation: cell adhesion and cell division. Alignment of these mechanisms in space allows for redundancy in the system and ensures the development of a coherent epithelial structure within a growing organ.

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Liang, X., Weberling, A., Hii, C. Y., Zernicka-Goetz, M., Buckley, C. E.. 2021-12-01. CADHERIN mediated AMIS localisation. https://doi.org/10.1101/2021.11.30.470571

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