Hepatocytes with a phenotype of substantial CYP3A4 induction generated from drug-associated fulminant hepatitis-derived induced pluripotent stem cells

Ite, K.; Toyoda, M.; Yoshioka, S.; Yukitake, T.; Yamazaki-Inoue, M.; Tatsumi, K.; Akutsu, H.; Nishina, H.; Kimura, T.; Ohtani, N.; Nakazawa, A.; Kasahara, M.; UMEZAWA, A.

doi:10.1101/2021.11.30.470519

bioRxiv · 10.1101/2021.11.30.470519

Hepatocytes with a phenotype of substantial CYP3A4 induction generated from drug-associated fulminant hepatitis-derived induced pluripotent stem cells

Ite, K.·Toyoda, M.·Yoshioka, S.·Yukitake, T.·Yamazaki-Inoue, M.·Tatsumi, K.·Akutsu, H.·Nishina, H.·Kimura, T.·Ohtani, N.·Nakazawa, A.·Kasahara, M.·UMEZAWA, A.

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Abstract

Hepatocyte-like cells (HLCs) generated from human pluripotent stem cells (PSCs) exhibit hepatocytic properties in vitro; however, their engraftment and functionality in vivo remain unsatisfactory. Despite optimization of differentiation protocols, HLCs did not engraft in a mouse model of liver injury. In contrast, organ-derived hepatocytes reproducibly formed colonies in the liver injury mouse model. As an extension of the phenomenon observed in hematopoietic stem cells giving rise to colonies within the spleen, commonly referred to as "colony-forming units in spleen (CFU-s", we hypothesize that "colony-forming units in liver (CFU-L)" serves as a reliable indicator of stemness, engraftment, and functionality of hepatocytes. The uniform expression of the randomly inactivated gene in a single colony, as reported by Sugahara et al. 2022, suggests that the colonies generated by isolated hepatocytes likely originate from a single cell. We, therefore, propose that CFU-L can be used to quantify the number of "hepatocytes that engraft and proliferate in vivo" as a quantitative assay for stem cells that utilize colony-forming ability, similar to that observed in hematopoietic stem cells.

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Ite, K., Toyoda, M., Yoshioka, S., Yukitake, T., Yamazaki-Inoue, M., Tatsumi, K., Akutsu, H., Nishina, H., Kimura, T., Ohtani, N., Nakazawa, A., Kasahara, M., UMEZAWA, A.. 2021-11-30. Hepatocytes with a phenotype of substantial CYP3A4 induction generated from drug-associated fulminant hepatitis-derived induced pluripotent stem cells. https://doi.org/10.1101/2021.11.30.470519

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