Convergent evolution of mevalonate pathway in Inonotus obliquus and Betula pendula.

Safronov, O.; Lutfullahoglubal, G.; Sipari, N.; Wilkens, M.; Safdari, P.; Smolander, O.-P.; Lihavainen, J.; Silvan, N.; Rajaraman, S.; Laine, P. K.; Paulin, L.; Auvinen, P.; Sarjala, T.; Overmyer, K.; Kangasjarvi, J.; Battersby, B.; Richter, U.; Salojarvi, J.

doi:10.1101/2021.11.28.470225

bioRxiv · 10.1101/2021.11.28.470225

Convergent evolution of mevalonate pathway in Inonotus obliquus and Betula pendula.

Safronov, O.·Lutfullahoglubal, G.·Sipari, N.·Wilkens, M.·Safdari, P.·Smolander, O.-P.·Lihavainen, J.·Silvan, N.·Rajaraman, S.·Laine, P. K.·Paulin, L.·Auvinen, P.·Sarjala, T.·Overmyer, K.·Kangasjarvi, J.·Battersby, B.·Richter, U.·Salojarvi, J.

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Abstract

Inonotus obliquus, Chaga mushroom, is a fungal species from Hymenochaetaceae family (Basidiomycota) which has been widely used for traditional medicine in Europe and Asia. Here, chaga genome was sequenced using Pacbio sequencing into a 50.7Mbp assembly consisting of 301 primary contigs with an N50 value of 375 kbp. Genome evolution analyses revealed a lineage-specific whole genome duplication event and an expansion of Cytochrome P450 superfamily. Fungal biosynthetic clusters were enriched for tandemly duplicated genes, suggesting that biosynthetic pathway evolution has proceeded through small-scale duplications. Metabolomic fingerprinting confirmed a highly complex terpene biosynthesis chemistry when compared against related fungal species lacking the genome duplication event.

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Safronov, O., Lutfullahoglubal, G., Sipari, N., Wilkens, M., Safdari, P., Smolander, O.-P., Lihavainen, J., Silvan, N., Rajaraman, S., Laine, P. K., Paulin, L., Auvinen, P., Sarjala, T., Overmyer, K., Kangasjarvi, J., Battersby, B., Richter, U., Salojarvi, J.. 2021-11-28. Convergent evolution of mevalonate pathway in Inonotus obliquus and Betula pendula.. https://doi.org/10.1101/2021.11.28.470225

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