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bioRxiv · 10.1101/2021.11.18.469135

Automated assembly scaffolding elevates a new tomato system for high-throughput genome editing

Alonge, M.·Lebeigle, L.·Kirsche, M.·Aganezov, S.·Wang, X.·Lippman, Z. B.·Schatz, M. C.·Soyk, S.
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Abstract

Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M82 along with Sweet-100, a rapid-cycling genotype that we developed to accelerate functional genomics and genome editing. This work outlines strategies to rapidly expand genetic systems and genomic resources in other plant species.

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Alonge, M., Lebeigle, L., Kirsche, M., Aganezov, S., Wang, X., Lippman, Z. B., Schatz, M. C., Soyk, S.. 2021-11-19. Automated assembly scaffolding elevates a new tomato system for high-throughput genome editing. https://doi.org/10.1101/2021.11.18.469135

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