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bioRxiv · 10.1101/2021.11.07.467596

An environmental control box for serial crystallography enables multi-dimensional experiments

Mehrabi, P.·von Stetten, D.·Leimkohl, J.-P.·Tellkamp, F.·Schulz, E. C.
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Abstract

We present an environmental enclosure for fixed-target serial crystallography, enabling X-ray diffraction experiments in a temperature window from below 10 {degrees}C to above 70 {degrees}C - a universal parameter of protein function. Via 5D-SSX time-resolved experiments can now be carried out at physiological temperatures, providing fundamentally new insights into protein function. We show temperature-dependent modulation of turnover kinetics for the mesophilic {beta}-lactamase CTX-M-14 and for the hyperthermophilic enzyme xylose isomerase.

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Mehrabi, P., von Stetten, D., Leimkohl, J.-P., Tellkamp, F., Schulz, E. C.. 2021-11-07. An environmental control box for serial crystallography enables multi-dimensional experiments. https://doi.org/10.1101/2021.11.07.467596

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