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bioRxiv · 10.1101/2021.11.05.467489

AN ECONOMIC, SQUARE-SHAPED FLAT-FIELD ILLUMINATION MODULE FOR TIRF-BASED SUPER-RESOLUTION MICROSCOPY

Lam, J. Y. L.·Wu, Y.·Dimou, E.·Zhang, Z.·Cheetham, M. R.·Koerbel, M.·Xia, Z.·Klenerman, D.·Danial, J. S. H.
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Abstract

Super-resolution (SR) microscopy allows complex biological assemblies to be observed with remarkable resolution. However, the presence of uneven Gaussian-shaped illumination hinders its use in quantitative imaging or high-throughput assays. Methods developed to circumvent this problem are often expensive, hard-to-implement, or not applicable to total internal reflection fluorescence (TIRF) imaging. We herein demonstrate a cost-effective method to overcome these challenges using a small square-core multimodal optical fibre as the coupler. We characterise our method with synthetic, recombinant and cellular systems imaged under TIRF and highly inclined and laminated optical sheet (HILO) illuminations to demonstrate its ability to produce highly uniform images under all conditions.

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Lam, J. Y. L., Wu, Y., Dimou, E., Zhang, Z., Cheetham, M. R., Koerbel, M., Xia, Z., Klenerman, D., Danial, J. S. H.. 2021-11-08. AN ECONOMIC, SQUARE-SHAPED FLAT-FIELD ILLUMINATION MODULE FOR TIRF-BASED SUPER-RESOLUTION MICROSCOPY. https://doi.org/10.1101/2021.11.05.467489

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