Motor unit recruitment strategies of the vastus lateralis according to sex

Guo, Y.; Jones, E. J.; Inns, T. B.; Ely, I. A.; Stashuk, D. W.; Wilkinson, D. J.; Smith, K.; Piasecki, J.; Phillips, B. E.; Atherton, P. J.; Piasecki, M.

doi:10.1101/2021.11.01.466761

bioRxiv · 10.1101/2021.11.01.466761

Motor unit recruitment strategies of the vastus lateralis according to sex

Guo, Y.·Jones, E. J.·Inns, T. B.·Ely, I. A.·Stashuk, D. W.·Wilkinson, D. J.·Smith, K.·Piasecki, J.·Phillips, B. E.·Atherton, P. J.·Piasecki, M.

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Abstract

AimDespite men exhibiting greater muscle strength and fatigibility than women, it remains unclear if there are sex-based differences in muscle recruitment strategies e.g. motor unit (MU) recruitment and modulation of firing rate (FR) at normalised forces and during progressive increases in force. MethodsTwenty-nine healthy male and thirty-one healthy female participants (18-35 years) were studied. Intramuscular electromyography was used to record individual motor unit potentials (MUPs) and near fibre MUPs from the vastus lateralis (VL) during 10% and 25% maximum isometric voluntary contractions (MVC), and spike-triggered averaging was used to obtain motor unit number estimates (MUNE) of the VL. Multilevel mixed-effects linear regression models were used to investigate the effects of sex at each contraction level. ResultsMen exhibited greater muscle strength (p<0.001) and size (p<0.001) than women, with no difference in force steadiness at 10% or 25% MVC. Women had smaller MUs and higher FR at 10% MVC (both p<0.02), similar to that at 25% MVC in MU size (p=0.062) and FR (p=0.031). However, both sexes showed similar increases in MU size and FR when moving from low-to mid-level contractions. There were no sex differences in any near fibre MUP parameters or in MUNE. ConclusionIn the vastus lateralis, women produce muscle force via different neuromuscular recruitment strategies to men which is characterised by smaller MUs discharging at higher rates. However, similar strategies are employed to increase force production from low to moderate contractions. These findings of similar proportional increases between sexes support the use of mixed sex cohorts in studies of this nature. Key pointsO_LIIncreases in muscle force production are mediated by motor unit (MU) recruitment, and MU firing rate (FR). C_LIO_LIWomen are underrepresented in studies of human neuromuscular research and markedly differ to men in a number of aspects of neuromuscular function, yet little is known of the recruitment strategies of each. C_LIO_LIHere we demonstrate men and women have similar vastus lateralis MU number estimates, yet women recruit smaller MUs with higher FR than men at normalised contraction levels. However, increases in force are achieved via similar trajectories of MU recruitment and MU FR in men and women. C_LIO_LIAlthough men and women exhibit divergent neuromuscular recruitment strategies to achieve normalised forces, increases in force are achived similarly and support the inclusion of mixed sex cohorts in studies of this nature. C_LI

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Guo, Y., Jones, E. J., Inns, T. B., Ely, I. A., Stashuk, D. W., Wilkinson, D. J., Smith, K., Piasecki, J., Phillips, B. E., Atherton, P. J., Piasecki, M.. 2021-11-03. Motor unit recruitment strategies of the vastus lateralis according to sex. https://doi.org/10.1101/2021.11.01.466761

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