Open-Source Low-Cost Cardiac Optical Mapping System

Rybashlykov, D.; Brennan, J.; Lin, Z.; Efimov, I. R.; Syunyaev, R.

doi:10.1101/2021.10.15.464535

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bioRxiv · 10.1101/2021.10.15.464535

Open-Source Low-Cost Cardiac Optical Mapping System

Rybashlykov, D.·Brennan, J.·Lin, Z.·Efimov, I. R.·Syunyaev, R.

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Abstract

Fluorescent imaging with voltage- or calcium-sensitive dyes, i.e. optical mapping, is one of the indispensable modern techniques to study cardiac electrophysiology, unsurpassed by temporal and spatial resolution. High-speed CMOS cameras capable of optical registration of action potential propagation are in general very costly. We present a complete solution priced below US$ 1,000 (including camera and lens) at the moment of publication with an open-source image acquisition and processing software. We demonstrate that the iDS UI-3130CP rev.2 camera we used in this study is capable of 200x200 977 frames per second (FPS) action potential recordings from rodent hearts. The signal-to-noise-ratio of a conditioned signal was 16 {+/-} 10 for rodent hearts. A comparison with a specialized MiCAM Ultimate-L camera has shown that signal-to-noise ratio (SNR) is sufficient for accurate measurements of AP waveform, conduction velocity ({+/-} 0.04 m/s) and action potential duration ({+/-} 7ms) in mouse and rat hearts. We measured the action potential prolongation during 4-aminopyridine administration in mouse heart, showing that proposed system signal quality is adequate for drug studies.

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Rybashlykov, D., Brennan, J., Lin, Z., Efimov, I. R., Syunyaev, R.. 2021-10-15. Open-Source Low-Cost Cardiac Optical Mapping System. https://doi.org/10.1101/2021.10.15.464535

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