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bioRxiv · 10.1101/2021.10.11.463917

Secondary structure of subgenomic RNA M of SARS-CoV-2

Soszynska-Jozwiak, M.·Kierzek, R.·Kierzek, E.
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Abstract

SARS-CoV-2 belongs the Coronavirinae family. As other coronaviruses, SARS-CoV-2 is enveloped and possesses positive-sense, single-stranded RNA genome of [~] 30 kb. Genome RNA is used as the template for replication and transcription. During these processes, positive-sense genomic RNA (gRNA) and subgenomic RNAs (sgRNAs) are created. Several studies showed importance of genomic RNA secondary structure in SARS-CoV-2 replication. However, the structure of sgRNAs have remained largely unsolved so far. In this study, we performed probing of sgRNA M of SARS-CoV-2 in vitro. This is the first experimentally informed secondary structure model of sgRNA M, which presents features likely to be important in sgRNA M function. The knowledge about sgRNA M provides insights to better understand virus biology and could be used for designing new therapeutics.

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Soszynska-Jozwiak, M., Kierzek, R., Kierzek, E.. 2021-10-12. Secondary structure of subgenomic RNA M of SARS-CoV-2. https://doi.org/10.1101/2021.10.11.463917

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