Rhythmicity of Intestinal IgA Responses Confers Oscillatory Commensal Microbiota Mutualism

Penny, H. A.; Domingues, R. G.; Krauss, M. Z.; Melo-Gonzalez, F.; Dickson, S.; Parkinson, J.; Hurry, M.; Purse, C.; Jegham, E.; Godinho-Silva, C.; Rendas, M.; Veiga-Fernandes, H.; Bechtold, D.; Grencis, R. K.; Toellner, K.-M.; Waisman, A.; Swann, J. R.; Gibbs, J. E.; Hepworth, M. R.

doi:10.1101/2021.10.11.463908

bioRxiv · 10.1101/2021.10.11.463908

Rhythmicity of Intestinal IgA Responses Confers Oscillatory Commensal Microbiota Mutualism

Penny, H. A.·Domingues, R. G.·Krauss, M. Z.·Melo-Gonzalez, F.·Dickson, S.·Parkinson, J.·Hurry, M.·Purse, C.·Jegham, E.·Godinho-Silva, C.·Rendas, M.·Veiga-Fernandes, H.·Bechtold, D.·Grencis, R. K.·Toellner, K.-M.·Waisman, A.·Swann, J. R.·Gibbs, J. E.·Hepworth, M. R.

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Abstract

Mutualistic interactions with the commensal microbiota are enforced through a range of immune responses that confer metabolic benefits for the host and ensure tissue health and homeostasis. Immunoglobulin (Ig)A responses directly determine the composition of commensal species that colonize the intestinal tract but require significant metabolic resources to fuel antibody production by tissue-resident plasma cells. Here we demonstrate IgA responses are subject to diurnal regulation by dietary-derived metabolic cues and a cell-intrinsic circadian clock. Rhythmicity in IgA secretion conferred oscillatory patterns on the commensal microbial community and its associated metabolic activity, resulting in changes to metabolite availability over the course of the circadian day. Our findings suggest circadian networks comprising intestinal IgA, the diet and the microbiota align to ensure metabolic health. One-Sentence SummaryWe demonstrate diurnal rhythms in intestinal IgA act to cross-regulate oscillations in the abundance of commensal microbes to foster mutualism.

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Penny, H. A., Domingues, R. G., Krauss, M. Z., Melo-Gonzalez, F., Dickson, S., Parkinson, J., Hurry, M., Purse, C., Jegham, E., Godinho-Silva, C., Rendas, M., Veiga-Fernandes, H., Bechtold, D., Grencis, R. K., Toellner, K.-M., Waisman, A., Swann, J. R., Gibbs, J. E., Hepworth, M. R.. 2021-10-12. Rhythmicity of Intestinal IgA Responses Confers Oscillatory Commensal Microbiota Mutualism. https://doi.org/10.1101/2021.10.11.463908

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