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bioRxiv · 10.1101/2021.09.22.461415

Peptide fusion improves prime editing efficiency

Velimirovic, M.·Zanetti, L.·Shen, M. W.·Fife, J. D.·Lin, L.·Cha, M.·Akinci, E.·Barnum, D.·Yu, T.·Sherwood, R. I.
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Abstract

Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. We present a high-throughput approach, PepSEq, to measure how fusion of 12,000 85-amino acid peptides derived from human DNA repair-related proteins influences prime editing efficiency. We show that peptide fusion can enhance prime editing, prime-enhancing peptides combine productively, and a top dual peptide-prime editor increases prime editing significantly in multiple cell lines across dozens of target sites.

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Velimirovic, M., Zanetti, L., Shen, M. W., Fife, J. D., Lin, L., Cha, M., Akinci, E., Barnum, D., Yu, T., Sherwood, R. I.. 2021-09-23. Peptide fusion improves prime editing efficiency. https://doi.org/10.1101/2021.09.22.461415

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