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bioRxiv · 10.1101/2021.09.22.460392

CAR T cell therapy drives endogenous locoregional T cell dynamics in a responding patient with glioblastoma

Abstract

CAR T cell therapy has transformed clinical care and management of patients with certain hematological cancers. However, it remains unclear whether the success of CAR T cell therapy relies solely on CAR T cell engagement with tumor antigen, or if it also requires the stimulation of an individual patients endogenous T cell response. Here, we performed combined analysis of longitudinal, single cell RNA and T cell receptor sequencing on glioblastoma tumors, peripheral blood (PB), and cerebrospinal fluid (CSF) from a patient with recurrent multifocal glioblastoma that underwent a remarkable response followed by recurrence on IL13RA2-targeted CAR T cell therapy (Brown et al. 2016). Single cell analysis of a tumor resected prior to CAR T cell therapy revealed the existence of an inflamed tumor microenvironment including a CD8+ cytotoxic, clonally expanded and antigen specific T cell population that disappeared in the recurrent setting. Longitudinal tracking of T cell receptors uncovered distinct T cell dynamics classes in the CSF during CAR T cell therapy. These included T cell clones with transient dynamics, representing intraventricular CAR T cell delivery and endogenous T cell recruitment from the PB into the CSF; and a group of T cells in the cerebrospinal fluid, that tracked with clonally expanded tumor resident T cells and whose dynamics contracted concomitantly with tumor volume. Our results suggest the existence of an endogenous T cell population that was invigorated by intraventricular CAR T cell infusions, and combined with the therapy to produce a complete response.

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BibTeXRIS

Jonsson, V. D., Ng, R., Dullerud, N., Wong, R. A., Hibbard, J., Wang, D., Aguilar, B., Starr, R., Weng, L., Alizadeh, D., Forman, S., Badie, B., Brown, C. E.. 2021-09-23. CAR T cell therapy drives endogenous locoregional T cell dynamics in a responding patient with glioblastoma. https://doi.org/10.1101/2021.09.22.460392

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