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bioRxiv · 10.1101/2021.09.18.460850

Lysosome-related organelles contain an expansion compartment that mediates delivery of zinc transporters to promote homeostasis

Mendoza, A. D.·Dietrich, N.·Tan, C.-H.·Herrera, D. E.·Kasiah, J.·Payne, Z.·Schneider, D. L.·Kornfeld, K.
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Abstract

Lysosome-related organelles play evolutionarily conserved roles in zinc storage, but mechanisms that control zinc flow in and out are not well understood. In C. elegans intestinal cells, the CDF-2 transporter stores zinc in these organelles during excess. Here we identify ZIPT-2.3 as the transporter that releases zinc during deficiency. The expression levels of CDF-2 and ZIPT-2.3 are reciprocally regulated in zinc excess and deficiency, establishing a fundamental mechanism of homeostasis. Super-resolution microscopy demonstrated these organelles are composed of a spherical acidified compartment and a hemispherical expansion compartment. The expansion compartment inflates during zinc excess and deficiency by vesicle fusion delivering zinc transporters. These results identify an unexpected structural feature of lysosome-related organelles that facilitates rapid transitions in the composition of zinc transporters to mediate homeostasis.

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Mendoza, A. D., Dietrich, N., Tan, C.-H., Herrera, D. E., Kasiah, J., Payne, Z., Schneider, D. L., Kornfeld, K.. 2021-09-19. Lysosome-related organelles contain an expansion compartment that mediates delivery of zinc transporters to promote homeostasis. https://doi.org/10.1101/2021.09.18.460850

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