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bioRxiv · 10.1101/2021.09.17.460814

A BioID-derived proximity interactome for SARS-CoV-2 proteins

May, D. G.·Martin-Sancho, L.·Anschau, V.·Liu, S.·Chrisopulos, R. J.·Scott, K. L.·Halfmann, C. T.·Pena, R. D.·Pratt, D.·Campos, A. R.·Roux, K. J.
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Abstract

The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here we use BioID to map the SARS-CoV-2 virus-host interactome using human lung cancer derived A549 cells expressing individual SARS-CoV-2 viral proteins. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are in association with SARS-CoV-2 proteins. We have also established a website to host the proteomic data to allow for public access and continued analysis of host-viral protein associations and whole-cell proteomes of cells expressing the viral-BioID fusion proteins. Collectively, these studies provide a valuable resource to potentially uncover novel SARS-CoV-2 biology and inform development of antivirals.

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May, D. G., Martin-Sancho, L., Anschau, V., Liu, S., Chrisopulos, R. J., Scott, K. L., Halfmann, C. T., Pena, R. D., Pratt, D., Campos, A. R., Roux, K. J.. 2021-09-21. A BioID-derived proximity interactome for SARS-CoV-2 proteins. https://doi.org/10.1101/2021.09.17.460814

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