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bioRxiv · 10.1101/2021.09.01.458564

SHOOT: phylogenetic gene search and ortholog inference

Emms, D.·Kelly, S.
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Abstract

Determining the evolutionary relationships between genes is fundamental to comparative biological research. Here we present the phylogenetic search, SHOOT. SHOOT searches a user query sequence against a database of phylogenetic trees and returns a tree with the query sequence correctly placed within it. We show that SHOOT performs this analysis with comparable speed to a BLAST search. We demonstrate that SHOOT phylogenetic placements are as accurate as conventional tree inference and it can identify orthologs with high accuracy. In summary, SHOOT is a fast and accurate tool for phylogenetic analyses of novel query sequences. It is available online at www.shoot.bio.

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Emms, D., Kelly, S.. 2021-09-03. SHOOT: phylogenetic gene search and ortholog inference. https://doi.org/10.1101/2021.09.01.458564

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