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bioRxiv · 10.1101/2021.08.28.458011

Vectofusin-1 based T-cell transduction approach for developing murine CAR-T cells for cancer.

Patel, R.·Devashish, K.·Singh, S.·Nath, P. R.·Gohel, D. M.·Prasad, R.·Singh, M.·Saini, N. Y.
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Abstract

Gene transfer into human and murine T-cells using viral-based approaches has several promising therapeutic applications including the production of chimeric antigen receptor T-cell (CAR-T) therapy. The generation of murine CAR-T is paramount to test and validate immunocompetent mouse models for CAR-T therapy. Several viral transduction enhancers already exist for gene therapy with few limitations. In this study, we tested vectofusin-1, a short cationic peptide, as a soluble transduction enhancer for gammaretroviral transduction for the generation of anti-CD19 murine CAR-T. We found that in comparison to Retronectin, Vectofusin-1 is an equally optimal transduction enhancer for the generation of murine CAR-T cells.

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Patel, R., Devashish, K., Singh, S., Nath, P. R., Gohel, D. M., Prasad, R., Singh, M., Saini, N. Y.. 2021-08-28. Vectofusin-1 based T-cell transduction approach for developing murine CAR-T cells for cancer.. https://doi.org/10.1101/2021.08.28.458011

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