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bioRxiv · 10.1101/2021.08.12.456100

Tissue-wide metabolomics reveals wide impact of gut microbiota on mice metabolite composition

Zarei, I.·Koistinen, V. M.·Kokla, M.·Klavus, A.·Babu, A. F.·Lehtonen, M.·Auriola, S.·Hanhineva, K.
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Abstract

The essential role of gut microbiota in health and disease is well-recognized, but the biochemical details underling beneficial impact remain largely undefined. Dysbiosis of gut bacteria results in the alteration of certain microbial and host metabolites, and identifying these markers could enhance the early detection of certain diseases. We report LC-MS based non-targeted metabolic profiling to demonstrate a large effect of gut microbiota on mammalian tissue metabolites. It was hypothesized that gut microbiota influences the overall biochemistry of the host metabolome and this effect is tissue-specific. Thirteen different tissues from germ-free and conventional mice were selected and their metabolic differences were analyzed. Our study demonstrated a large effect of the microbiome on mammalian biochemistry at different tissue levels and resulted in significant modulation of metabolites from multiple metabolic pathway (p [≤] 0.05). A vast metabolic response of host to metabolites generated by the microbiota was observed, Hundreds of molecular features were detected exclusively in one mouse group, with the majority of these being unique to specific tissue, suggesting direct impact gut microbiota on host metabolism.

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Zarei, I., Koistinen, V. M., Kokla, M., Klavus, A., Babu, A. F., Lehtonen, M., Auriola, S., Hanhineva, K.. 2021-08-13. Tissue-wide metabolomics reveals wide impact of gut microbiota on mice metabolite composition. https://doi.org/10.1101/2021.08.12.456100

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