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bioRxiv · 10.1101/2021.07.28.454109

miR-17~92 exerts stage-specific effects in adult V-SVZ neural stem cell lineages

Favaloro, F.·DeLeo, A.·Delgado, A.·Doetsch, F.
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Abstract

In the adult mouse brain, neural stem cells (NSCs) in the ventricular-subventricular zone (V-SVZ) generate neurons and glia throughout life. microRNAs are important regulators of cell states, frequently acting in a stage- or context-dependent manner. Here, miRNA profiling of FACS-purified populations identified miR-17[~]92 as highly upregulated in activated NSCs and transit amplifying cells (TACs) in comparison to quiescent NSCs. Conditional deletion of miR-17[~]92 in NSCs reduced stem cell proliferation both in vitro and in vivo. In contrast, in TACs, miR-17[~]92 deletion caused a selective shift from neurogenic DLX2+ TACs towards oligodendrogenic OLIG2+ TACs, resulting in increased oligodendrogenesis to the corpus callosum. miR-17[~]92 deletion also decreased proliferation and maturation of intraventricular oligodendrocyte progenitor cells. Together, these findings reveal stage- and cell-type-specific functions of the miR-17[~]92 cluster within adult V-SVZ neural stem cell lineages.

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Favaloro, F., DeLeo, A., Delgado, A., Doetsch, F.. 2021-07-28. miR-17~92 exerts stage-specific effects in adult V-SVZ neural stem cell lineages. https://doi.org/10.1101/2021.07.28.454109

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