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bioRxiv · 10.1101/2021.07.14.452416

Increased intestinal permeability in an orally-reactive peanut allergy model identifies Angiopoietin like-4 as a biomarker

Steinbach, E. C.·Smeekens, J. M.·Roy, S.·Toyonaga, T.·Cornaby, C.·Perini, L. B.·Berglind, A. E.·Kulis, M. D.·Kim, E. H.·Ferris, M. T.·Furey, T. S.·Burks, A. W.·Sheikh, S. Z.
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Abstract

Peanut allergy reaction severity correlates with increased intestinal epithelial cell (IEC) barrier permeability. CC027/GeniUnc mice develop peanut allergy by intragastric administration of peanut proteins without adjuvant. We report that peanut-allergic CC027/GeniUnc mice showed increased IEC barrier permeability and systemic peanut allergen Ara h 2 after challenge. Jejunal epithelial cell transcriptomics showed effects of peanut allergy on IEC proliferation, survival, and metabolism, and revealed IEC-predominant angiopoietin like-4 (Angptl4) as a unique feature of CC027/GeniUnc peanut allergy. CC027/GeniUnc mice and peanut-allergic pediatric patients demonstrated significantly higher serum Angptl4 and ANGPTL4 compared to control C3H/HeJ mice and non-peanut-allergic but atopic patients, respectively, highlighting its potential as a biomarker of peanut allergy.

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Steinbach, E. C., Smeekens, J. M., Roy, S., Toyonaga, T., Cornaby, C., Perini, L. B., Berglind, A. E., Kulis, M. D., Kim, E. H., Ferris, M. T., Furey, T. S., Burks, A. W., Sheikh, S. Z.. 2021-07-15. Increased intestinal permeability in an orally-reactive peanut allergy model identifies Angiopoietin like-4 as a biomarker. https://doi.org/10.1101/2021.07.14.452416

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