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bioRxiv · 10.1101/2021.07.03.451006

Dopaminergic axon tracts within a hyaluronic acid hydrogel encasement for implantation to restore the nigrostriatal pathway

Gordian-Velez, W. J.·Browne, K. D.·Galarraga, J. H.·Duda, J. E.·Espana, R. A.·Chen, H. I.·Burdick, J. A.·Cullen, D. K.
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Abstract

Parkinsons disease (PD) affects 10 million patients worldwide, making it the second most prevalent neurodegenerative disease. Motor symptoms emerge from the loss of dopamine in the striatum after the death of dopaminergic neurons and the long-projecting axons of the nigrostriatal pathway. Current treatments, such as dopamine replacement, deep brain stimulation or cell therapies, disregard the loss of this pathway at the core of symptoms. We sought to address this by improving our tissue-engineered nigrostriatal pathway (TE-NSP) technology, which consists of a tubular hydrogel with a collagen/laminin core that encases an aggregate of dopaminergic neurons and their axons in a way that resembles the nigrostriatal pathway. These constructs can be implanted to replace the lost neurons and axons with fidelity to the pathway, and thus provide dopamine according to feedback from the host circuitry. While TE-NSPs have been traditionally fabricated with agarose, here we utilized a hyaluronic acid (HA) hydrogel to expand the functionality of the encasement and our control over its properties. Using rat ventral midbrain neurons, we found that TE-NSPs exhibited longer and faster neurite growth with HA relative to agarose, with no differences observed in electrically-evoked dopamine release. When transplanted, HA hydrogels reduced host neuron loss and inflammation around the implant compared to agarose, and the cells and axons within TE-NSPs survived and maintained their cytoarchitecture for at least 2 weeks. HighlightsO_LIWe fabricated engineered dopaminergic axons encased in a tubular hydrogel. C_LIO_LIWe made hydrogels from methacrylated hyaluronic acid and compared them to agarose. C_LIO_LIAxons in HA hydrogels had longer and faster axon growth and displayed evoked dopamine release. C_LIO_LIHA hydrogels reduced the host inflammatory response and supported neuron and axon survival in vivo. C_LIO_LIThis platform may be used to reconstruct the nigrostriatal pathway to treat Parkinsons disease. C_LI

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Gordian-Velez, W. J., Browne, K. D., Galarraga, J. H., Duda, J. E., Espana, R. A., Chen, H. I., Burdick, J. A., Cullen, D. K.. 2021-07-04. Dopaminergic axon tracts within a hyaluronic acid hydrogel encasement for implantation to restore the nigrostriatal pathway. https://doi.org/10.1101/2021.07.03.451006

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