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bioRxiv · 10.1101/2021.07.01.450699

Inverse design of cholesterol attracting transmembrane helices reveals a paradoxical role of hydrophobic length

Methorst, J.·van Hilten, N.·Risselada, H. J.
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Abstract

The occurrence of linear cholesterol-recognition motifs in alpha-helical transmembrane domains has long been debated. Here, we demonstrate the ability of a genetic algorithm guided by coarse-grained molecular dynamics simulations--a method coined evolutionary molecular dynamics (Evo-MD)--to directly resolve the sequence which maximally attracts cholesterol for single-pass alpha-helical transmembrane domains (TMDs). We illustrate that the evolutionary landscape of cholesterol attraction in membrane proteins is characterized by a sharp, well-defined global optimum. Surprisingly, this optimal solution features an unusual short, slender hydrophobic block surrounded by three successive lysines. Owing to the membrane thickening effect of cholesterol, cholesterol-enriched ordered phases favor TMDs characterized by a long rather than a too short hydrophobic length (a negative hydrophobic mismatch). However, this short hydrophobic pattern evidently offers a pronounced net advantage for the attraction of free cholesterol in both coarse-grained and atomistic simulations. We illustrate that optimal cholesterol attraction is in fact based on the superposition of two distinct structural features: (i) slenderness and (ii) hydrophobic mismatch. In addition, we explore the evolutionary occurrence and feasibility of the two features by analyzing existing databases of membrane proteins and through the direct expression of analogous short hydrophobic sequences in live cell assays. The puzzling sequence variability of proposed linear cholesterol-recognition motifs is indicative of a sub-optimal membrane-mediated attraction of cholesterol which markedly differs from ligand binding based on shape compatibility. Significance StatementOur work demonstrates how a synergy between evolutionary algorithms and high-throughput coarse-grained molecular dynamics can yield fundamentally new insights into the evolutionary fingerprints of protein-mediated lipid sorting. We illustrate that the evolutionary landscape of cholesterol attraction in isolated transmembrane domains is characterized by a well-defined global optimum. In contrast, sub-optimal attraction of cholesterol is associated with a diverse solution space and features a high sequence variability despite acting on the same unique molecule. The contrasting physicochemical nature of the resolved attraction optimum suggests that cholesterol attraction via linear motifs does not pose a dominant pressure on the evolution of transmembrane proteins.

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Methorst, J., van Hilten, N., Risselada, H. J.. 2021-07-01. Inverse design of cholesterol attracting transmembrane helices reveals a paradoxical role of hydrophobic length. https://doi.org/10.1101/2021.07.01.450699

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