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bioRxiv · 10.1101/2021.06.30.450603

Structural studies on cell wall biogenesis-I: A method to produce high-quality protoplasts from alga Micrasterias denticulata, an emerging model in plant biology

Selvaraj, M.
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Abstract

Micrasterias denticulate is a freshwater unicellular green alga emerging as a model system in plant cell biology. This is an algae that has been examined in the context of cell wall research from early 1970s. Protoplast production from such a model system is important for many downstream physiological and cell biological studies. The algae produce intact protoplast in a straight two-step protocol involving 5% mannitol, 2% cellulysin, 4mM calcium chloride under a temperature ramping strategy. The process of protoplast induction and behavior of protoplast was examined by light microscopy and reported in this study.

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Selvaraj, M.. 2021-07-01. Structural studies on cell wall biogenesis-I: A method to produce high-quality protoplasts from alga Micrasterias denticulata, an emerging model in plant biology. https://doi.org/10.1101/2021.06.30.450603

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