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bioRxiv · 10.1101/2021.06.25.449807

Gestationally-Dependent Immune Organization at the Maternal-Fetal Interface

Moore, A. R.·Vivanco Gonzalez, N.·Plummer, K. A.·Mitchel, O. R.·Kaur, H.·Rivera, M.·Collica, B.·Bendall, S. C.·Palmer, T. D.
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Abstract

The immune system and placenta have a dynamic relationship across gestation to accommodate fetal growth and development. High-resolution characterization of this maternal- fetal interface is necessary to better understand the immunology of pregnancy and its complications. We developed a single-cell framework to simultaneously immuno-phenotype circulating, endovascular, and tissue-resident cells at the maternal-fetal interface throughout gestation, discriminating maternal and fetal contributions. Our data reveal distinct immune profiles across the endovascular and tissue compartments with tractable dynamics throughout gestation that respond to a systemic immune challenge in a gestationally-dependent manner. We uncover that mononuclear phagocytes and neutrophils drive the temporal immune composition of the placenta with remarkably diverse populations, including PD-L1-expressing subsets having compartmental and early gestational bias. Our approach and accompanying datasets provide a resource for additional investigations into gestational immunology and evoke a more significant role for the innate immune system in establishing the microenvironment of early pregnancy. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=200 SRC="FIGDIR/small/449807v1_ufig1.gif" ALT="Figure 1"> View larger version (56K): org.highwire.dtl.DTLVardef@6235corg.highwire.dtl.DTLVardef@fba572org.highwire.dtl.DTLVardef@12b0af4org.highwire.dtl.DTLVardef@dc44da_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Moore, A. R., Vivanco Gonzalez, N., Plummer, K. A., Mitchel, O. R., Kaur, H., Rivera, M., Collica, B., Bendall, S. C., Palmer, T. D.. 2021-06-26. Gestationally-Dependent Immune Organization at the Maternal-Fetal Interface. https://doi.org/10.1101/2021.06.25.449807

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