ultraID: a compact and efficient enzyme for proximity-dependent biotinylation in living cells

Zhao, X.; Bitsch, S.; Kubitz, L.; Schmitt, K.; Deweid, L.; Roehrig, A.; Cappio Barazzone, E.; Valerius, O.; Kolmar, H.; Bethune, J.

doi:10.1101/2021.06.16.448656

bioRxiv · 10.1101/2021.06.16.448656

ultraID: a compact and efficient enzyme for proximity-dependent biotinylation in living cells

Zhao, X.·Bitsch, S.·Kubitz, L.·Schmitt, K.·Deweid, L.·Roehrig, A.·Cappio Barazzone, E.·Valerius, O.·Kolmar, H.·Bethune, J.

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Abstract

Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has established itself as a key technology to study protein-protein interactions in living cells. A widespread approach, BioID, uses an abortive variant of the E. coli BirA biotin protein ligase, a quite bulky enzyme with slow labeling kinetics. To improve PDB versatility and speed, various enzymes have been developed by different approaches. Here we present a novel small-size engineered enzyme: ultraID. We show its practical use to probe the interactome of Argonaute-2 after a 10 min labeling pulse and expression at physiological levels. Moreover, using ultraID, we provide a membrane-associated interactome of coatomer, the coat protein complex of COPI vesicles. To date, ultraID is the smallest and most efficient biotin ligase available for PDB and offers the possibility of investigating interactomes at a high temporal resolution.

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Zhao, X., Bitsch, S., Kubitz, L., Schmitt, K., Deweid, L., Roehrig, A., Cappio Barazzone, E., Valerius, O., Kolmar, H., Bethune, J.. 2021-06-16. ultraID: a compact and efficient enzyme for proximity-dependent biotinylation in living cells. https://doi.org/10.1101/2021.06.16.448656

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