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bioRxiv · 10.1101/2021.06.14.448315

Ultrafast Light Targeting for High-Throughput Precise Control of Neuronal Networks

Faini, G.·Molinier, C.·Telliez, C.·Tourain, C.·Forget, B. C.·Ronzitti, E.·Emiliani, V.
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Abstract

Understanding how specific sets of neurons fire and wire together during cognitive-relevant activity is one of the most pressing questions in neuroscience. Two-photon, single-cell resolution optogenetics based on holographic light-targeting approaches enables accurate spatio-temporal control of individual or multiple neurons. Yet, currently, the ability to drive asynchronous activity in distinct cells is critically limited to a few milliseconds and the achievable number of targets to several dozens. In order to expand the capability of single-cell optogenetics, we introduce an approach capable of ultra-fast sequential light targeting (FLiT), based on switching temporally focused beams between holograms at kHz rates. We demonstrate serial-parallel photostimulation strategies capable of multi-cell sub-millisecond temporal control and many-fold expansion of the number of activated cells. This approach will be important for experiments that require rapid and precise cell stimulation with defined spatio-temporal activity patterns and optical control of large neuronal ensembles.

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Faini, G., Molinier, C., Telliez, C., Tourain, C., Forget, B. C., Ronzitti, E., Emiliani, V.. 2021-06-14. Ultrafast Light Targeting for High-Throughput Precise Control of Neuronal Networks. https://doi.org/10.1101/2021.06.14.448315

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