Characterisation of the symbionts in the Mediterranean fruitfly gut

Darrington, M.; Leftwich, P. T.; Holmes, N. A.; Friend, L. A.; Clarke, N. V. E.; Worsley, S. F.; Margaritopoulos, J. T.; Hogenhout, S. A.; Hutchings, M. I.; Chapman, T.

doi:10.1101/2021.06.09.447743

bioRxiv · 10.1101/2021.06.09.447743

Characterisation of the symbionts in the Mediterranean fruitfly gut

Darrington, M.·Leftwich, P. T.·Holmes, N. A.·Friend, L. A.·Clarke, N. V. E.·Worsley, S. F.·Margaritopoulos, J. T.·Hogenhout, S. A.·Hutchings, M. I.·Chapman, T.

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Abstract

Symbioses between bacteria and their insect hosts can range from very loose associations through to obligate interdependence. While fundamental evolutionary insights have been gained from the in-depth study of obligate mutualisms, there is increasing interest in the evolutionary potential of flexible symbiotic associations between hosts and their gut microbiomes. Understanding relationships between microbes and hosts also offers the potential for exploitation for insect control. Here, we investigate the gut microbiome of a global agricultural pest, the Mediterranean fruitfly (Ceratitis capitata). We used 16S rRNA profiling to compare the gut microbiomes of laboratory and wild strains raised on different diets and from flies collected from various natural plant hosts. The results showed that medfly guts harbour a fairly simple microbiome, primarily determined by the larval diet in both wild and laboratory flies. However, regardless of the laboratory diet or natural plant host on which flies were raised, Klebsiella spp dominated the medfly microbiomes and resisted removal by antibiotic treatment. We sequenced the genome of the dominant putative Klebsiella spp (designated Medkleb) isolated from the gut of the Toliman wild type fruitfly strain. Genome-wide ANI analysis placed Medkleb within the K. oxytoca / michiganensis group. Molecular, sequence and phenotypic analyses supported its identity as K. oxytoca. Medkleb has a genome size (5 825 435 bp) which is 1.6 standard deviations smaller than the mean genome size of free-living Klebsiella spp, and lacks some genes involved in environmental sensing. Moreover, the Medkleb genome contains at least two recently acquired unique genomic islands as well as genes that encode pectinolytic enzymes capable of degrading plant cell walls. This may be advantageous given that the medfly diet includes unripe fruits containing high proportions of pectin. These results suggest that the medfly harbours a commensal gut bacterium that may have developed a mutualistic association with its host and provide nutritional benefits.

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Darrington, M., Leftwich, P. T., Holmes, N. A., Friend, L. A., Clarke, N. V. E., Worsley, S. F., Margaritopoulos, J. T., Hogenhout, S. A., Hutchings, M. I., Chapman, T.. 2021-06-10. Characterisation of the symbionts in the Mediterranean fruitfly gut. https://doi.org/10.1101/2021.06.09.447743

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