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bioRxiv · 10.1101/2021.05.31.446496

CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments

Guo, J.·He, J.·Dehesh, K.·Yang, Z.
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Abstract

As a universal second messenger, calcium (Ca2+) transmits specific cellular signals via a spatiotemporal signature generated from its extracellular source and internal stores. Our knowledge of the mechanisms underlying generation of a Ca2+ signature is hampered by limited tools enabling simultaneous monitoring of the dynamics of Ca2+ levels in multiple subcellular compartments. To overcome the limitation and to further improve spatiotemporal resolutions, here we have assembled a molecular toolset (the CamelliA lines) in Arabidopsis that enables simultaneous and high-resolution monitoring of Ca2+ dynamics in multiple subcellular compartments through imaging analyses of different single-colored GECIs (Genetically Encoded Calcium Indicators). Indeed, the uncovering of the previously unrecognized Ca2+ signatures in three types of Arabidopsis cells in response to internal and external cues is a testimony to the wide applicability of the newly generated toolset for elucidating the subcellular sources contributing to the Ca2+signatures in plants. One sentence summaryA toolset for simultaneous imaging of Ca2+ dynamics in subcellular compartments has uncovered unrecognized Ca2+ signatures in Arabidopsis cells in response to developmental and external cues.

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Guo, J., He, J., Dehesh, K., Yang, Z.. 2021-06-01. CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments. https://doi.org/10.1101/2021.05.31.446496

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