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bioRxiv · 10.1101/2021.05.31.446394

Transcriptional characterization of the glial response due to chronic neural implantation of flexible microprobes

Joseph, K.·Kirsch, M.·Johnston, M.·Muenkel, C.·Stieglitz, T.·Haas, C. A.·Hofmann, U. G.
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Abstract

Long term implantation of (micro-)probes into neural tissue cause unique and disruptive responses to these foreign bodies. In this study, we present the transcriptional trajectory of glial cells responding to chronic implantation of flexible micro-probes for up to 18 weeks. Transcriptome analysis shows a rapid activation of microglial cells and a strong upregulation of reactive astrocytic genes, which is lost over the full duration of the implant period. Most interestingly, animals that were implanted for 18 weeks show a transcriptional profile similar to non-implanted controls, with increased expression of genes associated with wound healing and angiogenesis, which raises hope of a normalization of the neuropil to the pre-injury state when using flexible probes. Nevertheless, our data show, that a subset of genes upregulated after 18 weeks belong to the family of immediate early genes, which would indicate that structural and functional remodeling has not been completed at this time point. Our results confirm and extend previous work on the molecular changes resulting from the presence of intraneural probes and provide a rational basis for developing intervention strategies to control them.

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Joseph, K., Kirsch, M., Johnston, M., Muenkel, C., Stieglitz, T., Haas, C. A., Hofmann, U. G.. 2021-05-31. Transcriptional characterization of the glial response due to chronic neural implantation of flexible microprobes. https://doi.org/10.1101/2021.05.31.446394

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