Complete protection by a single dose skin patch delivered SARS-CoV-2 spike vaccine

McMillan, C. L. D.; Choo, J. J. Y.; Idris, A.; Supramaniam, A.; Modhiran, N.; Amarilla, A. A.; Isaacs, A.; Cheung, S. T. M.; Liang, B.; Bielefeldt-Ohmann, H.; Azuar, A.; Acharya, D.; Kelly, G.; Fernando, G. J. P.; Landsberg, M. J.; Khromykh, A. A.; Watterson, D.; Young, P. R.; McMillan, N. A. J.; Muller, D. A.

doi:10.1101/2021.05.30.446357

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bioRxiv · 10.1101/2021.05.30.446357

Complete protection by a single dose skin patch delivered SARS-CoV-2 spike vaccine

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Abstract

SARS-CoV-2 has infected over 160 million people and resulted in more than 3.3 million deaths, and we still face many challenges in the rollout of vaccines. Here, we use the high-density microarray patch to deliver a SARS-CoV-2 spike subunit vaccine directly to the skin. We show the vaccine, dry-coated on the patch is thermostable, and delivery of spike via HD-MAP induced greater cellular and antibody immune responses, with serum able to potently neutralize clinically relevant isolates including those from the B.1.1.7 and B.1.351 lineages. Finally, a single dose of HD-MAP-delivered spike provided complete protection from a lethal virus challenge, demonstrating that HD-MAP delivery of a SARS-CoV-2 vaccine is superior to traditional needle-and-syringe vaccination and has the potential to greatly impact the ongoing COVID-19 pandemic.

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McMillan, C. L. D., Choo, J. J. Y., Idris, A., Supramaniam, A., Modhiran, N., Amarilla, A. A., Isaacs, A., Cheung, S. T. M., Liang, B., Bielefeldt-Ohmann, H., Azuar, A., Acharya, D., Kelly, G., Fernando, G. J. P., Landsberg, M. J., Khromykh, A. A., Watterson, D., Young, P. R., McMillan, N. A. J., Muller, D. A.. 2021-05-31. Complete protection by a single dose skin patch delivered SARS-CoV-2 spike vaccine. https://doi.org/10.1101/2021.05.30.446357

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