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bioRxiv · 10.1101/2021.05.28.446194

In-Cell Sensitivity-Enhanced NMR of Intact Living Mammalian Cells

Ghosh, R.·Xiao, Y.·Kragelj, J.·Frederick, K. K.
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Abstract

NMR has the resolution and specificity to determine atomic-level protein structures of isotopically-labeled proteins in complex environments and, with the sensitivity gains conferred by dynamic nuclear polarization (DNP), NMR has the sensitivity to detect proteins at their endogenous concentrations. However, DNP sensitivity enhancements are critically dependent on experimental conditions and sample composition. While some of these conditions are theoretically compatible with cellular viability, the effects of others on cellular sample integrity are unknown. Uncertainty about the integrity of cellular samples limits the utility of experimental outputs. Using several measures, we establish conditions that support DNP enhancements that can enable detection of micromolar concentrations of proteins in experimentally tractable times that are compatible with cellular viability. Taken together, we establish DNP assisted MAS NMR as a technique for structural investigations of biomolecules in intact viable cells that can be phenotyped both before and after NMR experiments. ClassificationBiophysics and Structural Biology

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Ghosh, R., Xiao, Y., Kragelj, J., Frederick, K. K.. 2021-05-29. In-Cell Sensitivity-Enhanced NMR of Intact Living Mammalian Cells. https://doi.org/10.1101/2021.05.28.446194

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