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bioRxiv · 10.1101/2021.05.26.445844

Growth hormone transgenesis affects thermal tolerance in zebrafish (Danio rerio)

Almeida, D. V.·Figueiredo, M. A.·Marins, L. F.
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Abstract

In fish, growth hormone (GH)-transgenesis may modify physiological mechanisms of adaptation when challenged by biotic and abiotic stressors. Thus, we evaluated whether GH overexpression can alter the thermal tolerance of adult and juvenile GH-transgenic zebrafish (Danio rerio). This study compared the thermal tolerance in non-transgenic (NT) and GH-transgenic (T) zebrafish exposed to 13 {degrees}C, 39 {degrees}C, or 28 {degrees}C (control) for 96 h. Mortality rate was checked every 12 h in juvenile (8 week-old) and adult males (6 month-old). Exposure to different temperatures revealed that GH overexpression increases the tolerance of transgenic juveniles exposed to 13 {degrees}C and diminishes the tolerance of juveniles and adults, when exposed to 39 {degrees}C. Additionally, we have analyzed transcriptional expression from the heat shock proteins (HSPs), which are mainly involved in the thermal tolerance mechanism. The mRNA level analysis results revealed that, under controlled conditions (28 {degrees}C), GH-transgenesis upregulates the expression of hsp47, hsp70, hsp90a and heat shock transcription factor (hsf1a) in transgenic juveniles, although the same result was not observed in transgenic adults. Exposure to low temperature did not alter the expression of any analyzed gene, both in adults and in juveniles. Exposure to 39 {degrees}C decreased the expression of all genes analyzed, in GH-transgenic adults. Furthermore, the HSP expression pattern was analyzed via hierarchical clustering. This analysis revealed two major clusters illustrating the dependency of gene changes related to age. These results indicate that the GH overexpression can alter thermal tolerance of fish, depending of age and temperature. Highlights- GH-transgenesis increased the survival rate of juveniles at low temperature; - High temperature is more lethal for juvenile ande adult GH-transgenic zebrafish; - GH-transgenesis increased expression of hsf1a, hsp47, and hsp70 genes in juvenile zebrafish; - hsf1a, hsp47, hsp70, hsp90a, and hsp90b genes expression is diminished in adult zebrafish GH-transgenesis exposure at high temperature. O_FIG O_LINKSMALLFIG WIDTH=142 HEIGHT=200 SRC="FIGDIR/small/445844v1_ufig1.gif" ALT="Figure 1"> View larger version (59K): org.highwire.dtl.DTLVardef@1847289org.highwire.dtl.DTLVardef@7bb056org.highwire.dtl.DTLVardef@75efc9org.highwire.dtl.DTLVardef@d1a9ac_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Almeida, D. V., Figueiredo, M. A., Marins, L. F.. 2021-05-27. Growth hormone transgenesis affects thermal tolerance in zebrafish (Danio rerio). https://doi.org/10.1101/2021.05.26.445844

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