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bioRxiv · 10.1101/2021.05.26.445727

In vitro hepatotoxicity assessment of Lippia javanica (Burm.f.) Spreng. aqueous leaf extract

Swanepoel, B.·Koekemoer, T. C.·Venables, L.·Cloete, E.·Khumalo, N. P.·van de Venter, M.
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Abstract

Ethnopharmacological relevanceLippia javanica leaves are popular in traditional food, medicine and for insecticidal uses in various Africa countries and North-East India. Anecdotal evidence suggests that it is safe to use but limited animal studies suggested potential toxicity at high dosages, including hepatotoxicity. Aim of the studyTo screen for potential hepatotoxicity of L. javanica leaf extracts in vitro, thereby contributing to its toxicological profile for safe use in food and topical applications. Materials and methodsHigh content analysis techniques and fluorescent dyes were used to monitor C3A hepatocarcinoma cells for changes in morphological features that are associated with development of mitotoxicity, steatosis, oxidative stress, and lysosomal dysfunction. ResultsNo changes were observed in cell viability, reactive oxygen species or lysosomal content at concentrations up to 200 {micro}g/ml in C3A cells. Mitochondrial membrane potential was reduced by approximately 10% but this effect was not dose-dependent nor was it accompanied by a reduction in mitochondrial content. A dose-dependent decrease was observed in neutral lipid content. ConclusionThe results from this in vitro study suggest that L. javanica leaf extracts is not anticipated to be hepatotoxic at concentrations in the range that is assumed for food or topical use.

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Swanepoel, B., Koekemoer, T. C., Venables, L., Cloete, E., Khumalo, N. P., van de Venter, M.. 2021-05-27. In vitro hepatotoxicity assessment of Lippia javanica (Burm.f.) Spreng. aqueous leaf extract. https://doi.org/10.1101/2021.05.26.445727

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