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bioRxiv · 10.1101/2021.05.25.445679

The extracellular ATP receptor P2RX7 imprints a pro-memory transcriptional signature in effector CD8+ T cells

Vardam-Kaur, T.·van Dijk, S.·Peng, C.·Wanhainen, K. M.·Jameson, S. C.·Borges da Silva, H.
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Abstract

Development of central memory (Tcm) and resident memory (Trm) CD8+ T cells, which respectively promote immunity in the circulation and in barrier tissues, is not completely understood. Tcm and Trm cells may arise from common precursors, however their fate-inducing signals are elusive. We found that virus-specific effector CD8+ T cells display heterogeneous expression of the extracellular ATP sensor P2RX7. P2RX7-high expression is confined, at peak effector phase, to CD62L+ memory precursors which preferentially form Tcm cells. Among early effector CD8+ T cells, asymmetrical P2RX7 distribution correlated with distinct transcriptional signatures, with P2RX7-high cells enriched for memory and tissue-residency sets. P2RX7-high early effectors preferentially form both Tcm and Trm cells. Defective Tcm and Trm formation in P2RX7 deficiency is significantly reverted when the transcriptional repressor Zeb2 is ablated. Our study indicates that unequal P2RX7 upregulation in effector CD8+ T cells is a foundational element of the early Tcm/Trm fate.

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Vardam-Kaur, T., van Dijk, S., Peng, C., Wanhainen, K. M., Jameson, S. C., Borges da Silva, H.. 2021-05-25. The extracellular ATP receptor P2RX7 imprints a pro-memory transcriptional signature in effector CD8+ T cells. https://doi.org/10.1101/2021.05.25.445679

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