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bioRxiv · 10.1101/2021.05.25.445644

Locus-associated R-loop repression in cis

Khosraviani, N.·Abraham, K. J.·Chan, J. N. Y.·Mekhail, K.
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Abstract

R-loops exert varied beneficial or detrimental effects. To assess the function of an R-loop at a specific genetic locus, we had developed an inducible RNaseH1-EGFP-dCas9 (RED) protein chimaera as part of a locus-associated R-loop-modulating system (LasR). LasR is compatible with R-loop modulation in trans, which targets RED to one locus to repress R-loops at another spatially proximal site. Here we use the LasR system for R-loop modulation in cis, which consists of targeting RED directly to an R-loop. The combination of LasR in cis and trans will be essential to ascribe functions to specific R-loops within varied molecular contexts and study designs.

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Khosraviani, N., Abraham, K. J., Chan, J. N. Y., Mekhail, K.. 2021-05-25. Locus-associated R-loop repression in cis. https://doi.org/10.1101/2021.05.25.445644

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