In vivo direct imaging of neuronal activity at high temporo-spatial resolution

Toi, P. T.; Jang, H. J.; Min, K.; Kim, S.-P.; Lee, S.-K.; Lee, J.; Kwag, J.; Park, J.-Y.

doi:10.1101/2021.05.21.444581

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bioRxiv · 10.1101/2021.05.21.444581

In vivo direct imaging of neuronal activity at high temporo-spatial resolution

Toi, P. T.·Jang, H. J.·Min, K.·Kim, S.-P.·Lee, S.-K.·Lee, J.·Kwag, J.·Park, J.-Y.

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Abstract

There has been a longstanding demand for noninvasive neuroimaging methods capable of detecting neuronal activity at both high temporal and spatial resolution. Here, we propose a novel method that enables Direct Imaging of Neuronal Activity for functional MRI (termed DIANA-fMRI) that can dynamically image spiking activity in milliseconds precision, while retaining the original benefit of high spatial resolution of MRI. DIANA-fMRI was demonstrated through in vivo mice brain imaging at 9.4 T applying electrical whisker-pad stimulation, directly imaging the spiking activity as well as capturing its sequential propagation along the thalamocortical pathway, as further confirmed through in vivo spike recording and optogenetics. DIANA-fMRI will open up new avenues in brain science by providing a deeper understanding of the brains functional organization including neural networks.

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Toi, P. T., Jang, H. J., Min, K., Kim, S.-P., Lee, S.-K., Lee, J., Kwag, J., Park, J.-Y.. 2021-05-23. In vivo direct imaging of neuronal activity at high temporo-spatial resolution. https://doi.org/10.1101/2021.05.21.444581

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