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bioRxiv · 10.1101/2021.05.09.443220

SCON - A Short Conditional intrON for conditional knockout with one-step zygote injection

Wu, S.-H. S.·Szep-Bakonyi, R.·Lee, H.·Colozza, G.·Boese, A.·Gert, K. R.·Hallay, N.·Kim, J.·Zhu, Y.·Pilat-Carotta, S.·Theussl, H.-C.·Pauli, A.·Koo, B.-K.
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Abstract

The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables rapid generation of conditional alleles via one-step zygote injection. SCON has conditional intronic function in various vertebrate species and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

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Wu, S.-H. S., Szep-Bakonyi, R., Lee, H., Colozza, G., Boese, A., Gert, K. R., Hallay, N., Kim, J., Zhu, Y., Pilat-Carotta, S., Theussl, H.-C., Pauli, A., Koo, B.-K.. 2021-05-10. SCON - A Short Conditional intrON for conditional knockout with one-step zygote injection. https://doi.org/10.1101/2021.05.09.443220

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