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bioRxiv · 10.1101/2021.04.08.438850

The methyl phosphate capping enzyme Bin3 is a stable component of the fission yeast telomerase holoenzyme

Porat, J.·El Baidouri, M.·Grigull, J.·Deragon, J.-M.·Bayfield, M. A.
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Abstract

The telomerase holoenzyme is critical for maintaining eukaryotic genome integrity. In addition to a reverse transcriptase and an RNA template, telomerase contains additional proteins that protect the telomerase RNA and promote holoenzyme assembly. Here we report that the methyl phosphate capping enzyme (MePCE) Bin3 is a stable component of the S. pombe telomerase holoenzyme. Bin3 associates with the telomerase holoenzyme and U6 snRNA through an interaction with the recently described LARP7 family member Pof8, and we demonstrate that these two factors are evolutionarily linked in fungi. Our data suggest that the association of Bin3 with telomerase is independent of its methyltransferase activity, but rather that Bin3 functions in telomerase holoenzyme assembly by promoting TER1 accumulation and Pof8 recruitment to TER1. Taken together, this work yields new insight into the composition, assembly, and regulation of the telomerase holoenzyme in fission yeast as well as the breadth of its evolutionary conservation.

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Porat, J., El Baidouri, M., Grigull, J., Deragon, J.-M., Bayfield, M. A.. 2021-04-09. The methyl phosphate capping enzyme Bin3 is a stable component of the fission yeast telomerase holoenzyme. https://doi.org/10.1101/2021.04.08.438850

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