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bioRxiv · 10.1101/2021.04.02.438286

The chromatin remodeler Ino80 mediates alternative RNAPII pausing site determination

Cheon, Y.·Han, S.·Kim, T.·Lee, D.
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Abstract

Promoter-proximal pausing of RNA polymerase II (RNAPII) is a critical step in early transcription elongation for the precise regulation of gene expression. Here, we provide evidence of promoter-proximal pausing-like distributions of RNAPII in S. cerevisiae. We found that genes bearing an alternative pausing site utilize Ino80p to properly localize RNAPII pausing at the first pausing site and to suppress the accumulation of RNAPII at the second pausing site, which is tightly associated with the +1 nucleosome. This alternative pausing site determination was dependent on the remodeling activity of Ino80p to modulate the +1 nucleosome position and might be controlled synergistically with Spt4p. Furthermore, we observed similar Ino80-dependent RNAPII pausing in mouse embryonic stem cells (mESCs). Based on our collective results, we hypothesize that the chromatin remodeler Ino80 plays a highly conserved role in regulating early RNAPII elongation to establish intact pausing.

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Cheon, Y., Han, S., Kim, T., Lee, D.. 2021-04-03. The chromatin remodeler Ino80 mediates alternative RNAPII pausing site determination. https://doi.org/10.1101/2021.04.02.438286

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