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bioRxiv · 10.1101/2021.04.01.435439

Species and strain cultivation of skin, oral, and gut microbiota

Fleming, E.·Pabst, V.·Hoyt, A.·Zhou, W.·Hardy, R.·Peterson, A.·Beach, R.·Ondouah-Nzutchi, Y.·Dong, J.·Oh, J.
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Abstract

Genomics-driven discovery of microbial species have provided extraordinary insights into the biodiversity of human microbiota. High resolution genomics to investigate species- and strain-level diversity and mechanistic studies, however, rely on the availability of individual microbes from a complex microbial consortia. Here, we describe and validate a streamlined workflow for cultivating microbes from the skin, oral, and gut microbiota, informed by metagenomic sequencing, mass spectrometry, and strain profiling.

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Fleming, E., Pabst, V., Hoyt, A., Zhou, W., Hardy, R., Peterson, A., Beach, R., Ondouah-Nzutchi, Y., Dong, J., Oh, J.. 2021-04-01. Species and strain cultivation of skin, oral, and gut microbiota. https://doi.org/10.1101/2021.04.01.435439

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