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bioRxiv · 10.1101/2021.03.31.436355

Quantification of durable CRISPR-based gene silencing activity

Nakamura, M.·Ivec, A.·Gao, Y.·Qi, L. S.
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Abstract

Development of CRISPR-based technologies for regulating gene expression stands to provide novel methods for the study and engineering of biological behavior. New tools capable of inducing long-lasting changes in gene expression will increase the utility of these techniques, providing durable effects from one-time doses of reagents. We describe here a reporter system for quantifying the ability of CRISPR-based effectors to induce stable gene repression. We observe a continuous gradation of the ability of these effectors to silence gene expression, depending on the domain composition and configuration. We also report the creation of a single CRISPR protein capable of producing durable gene silencing. This assay should allow for the continued development of enhanced gene repression tools which will be useful in a wide array of biological research and engineering applications.

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Nakamura, M., Ivec, A., Gao, Y., Qi, L. S.. 2021-03-31. Quantification of durable CRISPR-based gene silencing activity. https://doi.org/10.1101/2021.03.31.436355

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