The proteome of remyelination is different from that of developmental myelination

Paes de Faria, J.; Azevedo, M. M.; Bausch-Fluck, D.; Seixas, A. I.; Domingues, H. S.; Monteiro, M. A.; Pedrioli, P. G.; Lopes, E.; Fernandes, R.; Zhao, C.; Franklin, R. J.; Wollscheid, B.; Relvas, J. B.; Montani, L.

doi:10.1101/2021.03.24.436006

bioRxiv · 10.1101/2021.03.24.436006

The proteome of remyelination is different from that of developmental myelination

Paes de Faria, J.·Azevedo, M. M.·Bausch-Fluck, D.·Seixas, A. I.·Domingues, H. S.·Monteiro, M. A.·Pedrioli, P. G.·Lopes, E.·Fernandes, R.·Zhao, C.·Franklin, R. J.·Wollscheid, B.·Relvas, J. B.·Montani, L.

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Abstract

Loss of myelin underlies the pathology of several neurological disorders of diverse etiology. CNS remyelination by adult oligodendrocyte progenitor cells (OPCs) can occur but it differs from developmental myelination carried out by neonatal OPCs. We asked whether the myelin proteome of remyelinated regions is changed. We compared the myelin proteome formed during development to the remyelination proteome attained after lysolecithin-induced demyelination in the mouse spinal cord. Mass-spectrometry analysis of iTRAQ labelled myelin protein lysates showed that the proteome of remyelination is different from that of developmental myelination, leading to profound changes in myelin protein content. Aside from known mediators of oligodendrocyte differentiation, we found proteome alterations included modulators of metabolism, cell signaling and actin cytoskeleton dynamics. Downregulating one candidate (FSCN1/Fascin1) was sufficient to partially hamper oligodendrocytes in-vitro. In summary, we identify the difference in the proteome of remyelinating oligodendrocytes as a novel potential contributor to the pathophysiology of demyelinating disorders, thus providing new potential therapeutic targets for future studies.

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Paes de Faria, J., Azevedo, M. M., Bausch-Fluck, D., Seixas, A. I., Domingues, H. S., Monteiro, M. A., Pedrioli, P. G., Lopes, E., Fernandes, R., Zhao, C., Franklin, R. J., Wollscheid, B., Relvas, J. B., Montani, L.. 2021-03-24. The proteome of remyelination is different from that of developmental myelination. https://doi.org/10.1101/2021.03.24.436006

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