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bioRxiv · 10.1101/2021.03.23.436577

Aggregated Mycobacterium tuberculosis enhances the inflammatory response

Abstract

Mycobacterium tuberculosis (Mtb) readily aggregates in culture and Mtb aggregates in the lung were observed in experimental Mtb infection. However, the physiological consequences of Mtb aggregation are incompletely understood. Here we examined the human macrophage transcriptional response to aggregated Mtb relative to infection with non-aggregated single or multiple bacilli per host cell. Infection with aggregated Mtb led to an early upregulation of pro-inflammatory associated genes and enhanced TNF signaling via the NF{kappa}B pathway. Both these pathways were significantly upregulated relative to infection with single bacilli, and TNF signaling was also significantly elevated relative to infection with multiple non-aggregated Mtb. Secretion of TNF and downstream cytokines were also enhanced. On a longer timescale, aggregate infection led to overall increased acidification per macrophage and a high proportion of death in these cells after aggregate phagocytosis. Host cell death did not occur when Mtb aggregates were heat killed despite such clumps being readily picked up. To validate that Mtb aggregates do occur in the human lung, we document Mtb aggregates surrounding a cavity in a human TB lesion. Aggregates may therefore be present in some lesions and elicit a stronger inflammatory response resulting in recruitment of additional phagocytes and their subsequent death, potentially leading to necrosis and transmission.

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Sigal, A., Rodel, H., Ferreira, I. M., Ziegler, C., Ganga, Y., Bernstein, M., Hwa, S.-H., Kaplan, G., Lustig, G., Nargan, K., Noursadeghi, M., Steyn, A., Shalek, A. K.. 2021-03-23. Aggregated Mycobacterium tuberculosis enhances the inflammatory response. https://doi.org/10.1101/2021.03.23.436577

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