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bioRxiv · 10.1101/2021.03.16.435675

FastTomo: A SerialEM Script for Collecting Electron Tomography Data

Xu, A.·Xu, C.
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Abstract

FastTomo is a SerialEM script for collecting tilted specimen images in transmission electron microscopes to be further used in tomographic reconstruction. It achieves a speedup over conventional tracking methods by minimizing the usage of off-target tracking shots, and instead applies proportional control to the specimen images. Movement in the Z coordinate is estimated prior to each tilt series in a separate calibration routine. Overall, this method is fast and reliable when the field of view is at least 1 um, and can tolerate minor errors in setting eucentric height. The implemented tilt series schemes include the unidirectional, bidirectional, and dose-symmetric schemes.

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Xu, A., Xu, C.. 2021-03-17. FastTomo: A SerialEM Script for Collecting Electron Tomography Data. https://doi.org/10.1101/2021.03.16.435675

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