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bioRxiv · 10.1101/2021.03.16.434153

BugSeq 16S: NanoCLUST with Improved Consensus Sequence Classification

Jung, A.·Chorlton, S. D.
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Abstract

NanoCLUST has enabled species-level taxonomic classification from noisy nanopore 16S sequencing data for BugSeqs users and the broader nanopore sequencing community. We noticed a high misclassification rate of NanoCLUST-derived consensus 16S sequences due to its use of BLAST top hit taxonomy assignment. We replaced the consensus sequence classifier of NanoCLUST with QIIME2s VSEARCH-based classifier to enable greater accuracy. We use mock microbial community and clinical 16S sequencing data to show that this replacement results in significantly improved nanopore 16S accuracy (over 5% recall and 19% precision), and make this new tool (BugSeq 16S) freely available for academic use at BugSeq.com/free.

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Jung, A., Chorlton, S. D.. 2021-03-17. BugSeq 16S: NanoCLUST with Improved Consensus Sequence Classification. https://doi.org/10.1101/2021.03.16.434153

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