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bioRxiv · 10.1101/2021.03.10.434724

Courtship ritual of male and female nuclei during fertilisation in Neurospora crassa

Abstract

Sexual reproduction is a key process influencing the evolution and adaptation of animals, plants and many eukaryotic microorganisms, such as fungi. Mycologists have described the different fungal fruiting bodies, while geneticists have partly unravelled the regulation of sexual development. However, the sequential fungal cell biology of fertilisation and the associated nuclear dynamics after plasmogamy are poorly understood. Using histone-fluorescent parental isolates, we tracked male and female nuclei during fertilisation in the model ascomycetes Neurospora crassa using live-cell-imaging. This study unravels the behaviour of trichogyne resident female nuclei and the extraordinary manner that male nuclei migrate up the trichogyne to the protoperithecium. Our observations raise new fundamental questions about the modus operandi of nuclei movements during sexual reproduction, male and female nuclear identity, guidance of nuclei within the trichogyne and, unexpectedly, the avoidance of "polyspermy" in fungi. The spatio-temporal dynamics of male nuclei within the trichogyne following plasmogamy are also described, where the speed and the deformation of male nuclei are of the most dramatic observed to date in a living organism.

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BibTeXRIS

Brun, S., Kuo, H.-C., Jeffree, C. E., Thomson, D. D., Read, N.. 2021-03-10. Courtship ritual of male and female nuclei during fertilisation in Neurospora crassa. https://doi.org/10.1101/2021.03.10.434724

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