A genetically encoded fluorescent biosensor for extracellular L-lactate

NASU, Y.; Murphy-Royal, C.; Wen, Y.; Haidey, J.; Molina, R. S.; Aggarwal, A.; Zhang, S.; Kamijo, Y.; Paquet, M.-E.; Podgorski, K.; Drobizhev, M.; Bains, J. S.; Lemieux, M. J.; Gordon, G. R.; Campbell, R. E.

doi:10.1101/2021.03.05.434048

bioRxiv · 10.1101/2021.03.05.434048

A genetically encoded fluorescent biosensor for extracellular L-lactate

NASU, Y.·Murphy-Royal, C.·Wen, Y.·Haidey, J.·Molina, R. S.·Aggarwal, A.·Zhang, S.·Kamijo, Y.·Paquet, M.-E.·Podgorski, K.·Drobizhev, M.·Bains, J. S.·Lemieux, M. J.·Gordon, G. R.·Campbell, R. E.

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Abstract

To enable investigations of the emerging roles of cell-to-cell shuttling of L-lactate, we have developed an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. We demonstrate that this biosensor, designated eLACCO1.1, enables minimally invasive cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.

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NASU, Y., Murphy-Royal, C., Wen, Y., Haidey, J., Molina, R. S., Aggarwal, A., Zhang, S., Kamijo, Y., Paquet, M.-E., Podgorski, K., Drobizhev, M., Bains, J. S., Lemieux, M. J., Gordon, G. R., Campbell, R. E.. 2021-03-06. A genetically encoded fluorescent biosensor for extracellular L-lactate. https://doi.org/10.1101/2021.03.05.434048

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