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bioRxiv · 10.1101/2021.03.01.433350

A Universal Chemical Method for Rational Design of Protein-based Nanoreactors

Reddy, M. M.·Bathla, P.·Sandanaraj, B. S.
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Abstract

Self-assembly of a monomeric protease to form a multi-subunit protein complex "proteasome" enables targeted protein degradation in living cells. The naturally occurring proteasomes serve as an inspiration and blueprint for the design of artificial protein-based nanoreactors. Here we disclose a general chemical strategy for the design of proteasome-like nanoreactors. Micelle-assisted protein labeling (MAPLab) technology along with the N-terminal bioconjugation strategy is utilized for the synthesis of a well-defined monodisperse self-assembling semi-synthetic protease. The designer protein is programmed to self-assemble into a proteasome-like nanostructure which preserves the functional properties of native protease.

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Reddy, M. M., Bathla, P., Sandanaraj, B. S.. 2021-03-01. A Universal Chemical Method for Rational Design of Protein-based Nanoreactors. https://doi.org/10.1101/2021.03.01.433350

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