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bioRxiv · 10.1101/2021.02.12.430999

An improved ChEC-seq method accurately maps the genome-wide binding of transcription coactivators and sequence-specific transcription factors

Donczew, R.·Lalou, A.·Devys, D.·Tora, L.·Hahn, S.
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Abstract

Mittal and colleagues have raised questions about mapping transcription factor locations on DNA using the MNase-based ChEC-seq method (Mittal et al., 2021). Partly due to this concern, we modified the experimental conditions of the MNase cleavage step and subsequent computational analyses, resulting in more stringent conditions for mapping protein-DNA interactions (Donczew et al., 2020). The revised method (dx.doi.org/10.17504/protocols.io.bizgkf3w) answers questions raised by Mittal et al. and, without changing earlier conclusions, identified widespread promoter binding of the transcription coactivators TFIID and SAGA at active genes. The revised method is also suitable for accurately mapping the genome-wide locations of DNA sequence-specific transcription factors.

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Donczew, R., Lalou, A., Devys, D., Tora, L., Hahn, S.. 2021-02-12. An improved ChEC-seq method accurately maps the genome-wide binding of transcription coactivators and sequence-specific transcription factors. https://doi.org/10.1101/2021.02.12.430999

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